几个影响黄瓜子叶体细胞胚胎发生的因素

Several Factors Affecting Somatic Embryos Derived from Cotyledons of Cucumber (Cucumis sativus)

黄瓜品种“叶三旱”是体细胞胚胎发生的理想基因型 ;愈伤组织诱导阶段和胚胎发生阶段分别采用 9%和 6 %的蔗糖浓度 ,可促进体细胞胚胎发生 ;胚诱导培养基中添加 6 BA 0 .5mg·L-1,体细胞胚胎发生率较高 (达到 6 7.8% ) ;愈伤组织诱导阶段甘露醇与蔗糖配合使用 ,可提高体细胞胚胎发生率 (高达 82 .2 % )。

Yesanhan' cucumber is an ideal genotype for somatic embryogenesis. The sucrose concentrations in embryogenic callus formation stage and somatic embryogenesis stage were 9% and 6% respectively, which promoted the frequency of somatic embryogenesis. When embryo inducing medium contained 6 BA at 0.5 mg·L -1 , the frequency of somatic embryogenesis reached 67.8%. Mannitol used together with sucrose at callus induction stage could increase the frequency of somatic embryogenesis to 82.2%.