期刊文献+

妊娠相关血浆蛋白A单克隆抗体的制备与鉴定 被引量:4

The preparation and identification for the monoclonal antibody against pregnancy associated plasma protein A
下载PDF
导出
摘要 建立妊娠相关血浆蛋白 A( PAPP-A)单克隆抗体细胞株 ,制备其单克隆抗体( Mc Ab) ,以用于检测孕妇血清中 PAPP-A的含量 ,提高先天性染色体异常疾病筛查的检出率。本室采用甲基纤维素半固体选择性培养基细胞融合技术获得了理想的 PAPP-A Mc Ab,为提高有关PAPP-A免疫分析技术的准确性。
出处 《生殖医学杂志》 CAS 2003年第3期170-174,共5页 Journal of Reproductive Medicine
  • 相关文献

参考文献11

  • 1徐志凯.实用单克隆抗体技术[M].西安:陕西科学技术出版社,1992.60-70.
  • 2Mckinney MM, Parkinson A. A simple nonchromatographic procedure to purify immunoglobulins from serum and ascites fluid [J]. J Immunol Med, 1987,96(2):271-278.
  • 3Lin TM, Galbert SP, Kiefer D, et al. Characterization of four human pregnancy-associated plasma proteins[J]. Am J Obstet Gynecol, 1974,118(2):223-236.
  • 4Oxvig C, Sand O, Kristensen T, et al. Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein[J]. J Biol Chem, 1993,268(17) : 12243-12246.
  • 5Conover CA, Oxvig C. Evidence that the insulin-like growth factor binding protein-A protease in human ovarian follicular fluid is pregnancy associated plasma protein-A[J]. J Clin Endocrinol Metab, 1999,84(12):4742-4745.
  • 6Hackshaw AK, Wald NJ. Assessment of the value of reporting partial Screening results in prenatal screening for Down syndrome [J]. Prenat Diagn, 2001,21 (9) :737-740.
  • 7Mauldin J. Prenatal diagnosis and fetal therapy:what lies in future[J]?Indian J Pediatr, 2000,67 (12):899-905.
  • 8Hackshaw AK, Wald NJ. Inaccurate estimation of risk in second trimester serum screening for Down syndrome among women who have already had first trimester screening [J]. Prenat Diagn, 2001,21 (9) :741-746.
  • 9Tsai MS, Huang YY, Hwa KY, et al. Combined measurement of fetal nuchal translucency, maternal serum free beta-hCG, and pregnancy-associated plasma protein A for first-trimester Down's syndrome screening[J]. J Formos Med Assoc, 2001,100(5):319-325.
  • 10Davis M, Pennington JE, Kubler AM, et al. A simple, single-step technique for selecting and cloning hybridomas for production of monoclonal antibodies[J]. J Immunol Methods, 1982,50(2) : 161.

共引文献57

同被引文献16

  • 1李龙,沈红,黄园园,陈静.氨苯磺胺免疫荧光分析方法的建立[J].动物医学进展,2007,28(8):17-20. 被引量:2
  • 2司徒镇强 吴军正.细胞培养[M].西安:世界图书版出版公司,2004.78-202.
  • 3曹泽毅.中华妇产科学[M].北京:人民卫生出版社,1999.2129.
  • 4Vladimir I,Muronetz,Timo K.lsolation of antigens and antibodies by affinity chromatography[J].Hromatography B,2003,790:53.
  • 5Richard R,Burgess,Nancy E.Thompson advances in gentle immunoaffinity chromatography[J].Cur Opin Biotechno,2002,13:304.
  • 6Murray A,Simms MS,Scholfield DP,et al.J Nucl Med,2001,42:726.
  • 7Cuckle HS,Vanlith JM.Appropriate biochemical parameters in first-trimester screening for Down syndrome[J].Prenat Diagn,1999,19(6):505-512.
  • 8Jauniaux E,Bersinger NA,Gulbis B,et al.The contribution of maternal serum markers in the early prenatal diagnosis of molar pregnancies[J].Hum Reprod,1999,14 (3):842-846.
  • 9Hotakainen K,Haglund C,Paju A,et al.Chorionic gonadotropin beta-subunit and core fragment in bladder cancer:mRNA and protein expression in urine,serum and tissue[J].Eur Urol,2002,41 (6):677-685.
  • 10Sun IY,Overgaard MT,Oxvig C,et al.Pregnancy-associated plasma protein A proteolytic activity is associated with the human placental trophoblast cell membrane[J].J Clin Endocrinol Metab,2002,87 (11):5235-5240.

引证文献4

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部