2-萘酚反相高效液相色谱分析

The Analysis of 2-Aaphthol by Reversed-phase HPLC

本文以反相高效液相色谱对2-萘酚及有机杂质进行分析。选择流动相,离子对,检测波长,并研究了不同牌号ODS C18色谱柱的重现性,得到了2-萘酚及杂质萘、1-萘酚、2,2’-联萘酚的相关系数分别为:0.9998;0.9990:0.9912;0.9979。标准偏差和变异系数分别为:0.2738％,0.2776％;0.00679％,1.4839％;0.00527％,1.2663％;0.007133％,1.7746％。得到了它们准确度的数据。并确定了最小检出量。

2-Naphthol and organic impurities were analyzed by reversed phase HPLC, the mobile phase, ion pair and determining wavelength were selected, the reproducibility of different trademark of ODS C18 was studied. In the best condition of separating, we can find the correlation coefficients of 2-naphthol, naphthalene, 1-naphthol, 2,2'-dinaphthol, were 0.9997,0.9990,0.9991,0.9979 the standard deviations of them were 0.2738%, 0.2776%; 0.00679%, 1.4839%; 0.00527%, 1.2663%; 0.00713%, 1.7746%, the coefficients of variation of them were, the data of rightness of them by experiment. We also assessed the minimum for determining.