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芬太尼对人外周血NF-κB活性的影响 被引量:1

Effects of fentanyl on activation nuclear factor-kappaB in human whole blood
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摘要 目的 验证芬太尼是否影响免疫炎症反应中的某些因素 ,如同吗啡。方法 外周血取自 7个正常志愿者 ,实验分为正常对照组、芬太尼 ( 2 0 μg·L-1和 2mg·L-1)组、模型组(脂多糖 ,LPS组 )和治疗组 (芬太尼 2 0 μg·L-1+LPS、芬太尼 2mg·L-1+LPS)。用流式细胞术检测人外周血中性粒细胞和单核细胞中核因子(NF κB)活性 ,用ELISA检测血清中肿瘤坏死因子 α(TNF α)和白介素 6(IL 6)含量。结果 芬太尼组NF κB活性及TNF α和IL 6含量与正常对照组比较 ,均无明显差异 (P >0 .0 5 )。治疗组 (芬太尼 2 0 μg·L-1+LPS、芬太尼 2mg·L-1+LPS)中NF κB的活性分别为 81 .9% ,76.1 % (中性粒细胞 )和 78.6% ,72 .6%(单核细胞) ,明显低于模型组 88.9%和 85 .1 % (P <0 .0 1 )。TNF α含量在治疗组 (芬太尼 2 0 μg·L-1+LPS、芬太尼 2mg·L-1+LPS)为 45 9和 3 5 7ng·L-1,IL 6为 796和 72 0ng·L-1,两者均低于模型组 (其中TNF α为 1 2 2 6ng·L-1,IL 6为 1 5 63ng·L-1) (P <0 .0 1 )。结论 芬太尼对NF κB的活性及TNF α和IL 6的含量无影响 ,但可抑制LPS诱导的NF κB的活性及TNF α和IL 6的含量 。 AIM To clarify if fentanyl affects some factors in immunity and inflammation as morphine did. METHODS Blood samples were collected from 7 healthy volunteers. Each was divided into 6 parts: normal control, fentanyl(20 μg·L -1 or 2 mg·L -1 ) control, lipopolysaccharide(LPS) alone and fentanyl 20 μg·L -1 or 2 mg·L -1 +LPS. The nuclear factor kappaB(NF κB) activation in human neutrophils and monocytes was examined by flow cytometric analysis, the plasma tumor necrosis factorα(TNF α) and interleukin 6(IL 6) concentrations were measured using ELISA. RESULTS The low NF κB activation (2.8%- 4.0%) and TNF α and IL 6 production was no significant difference in normal control and fentanyl control(P>0.05). The NF κB activation in treatment groups(fentanyl 20 μg· L -1 +LPS , fentanyl 2 mg·L -1 +LPS) were 81.9% and 76.1% in neutrophils and 78.6% and 72.6% in monocytes, respectively, which were less than those in LPS alone group(88.9% and 85.1%, P<0.01) . TNF α production in treatment groups(fentanyl 20 μg·L -1 +LPS, fentanyl 2 mg· L -1 +LPS) and LPS group were 459, 357 and 1226 ng·L -1 , IL 6 were 796, 720 and 1563 ng·L -1 , respectively. The differences were significant between treatment groups and LPS one (P<0.01) . CONCLUSION Fentanyl alone has no effect on NF κB activation and TNF α and IL 6 production, but attenuates LPS induced NF κB activation and TNF α and IL 6 production.
出处 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2003年第3期192-195,共4页 Chinese Journal of Pharmacology and Toxicology
基金 国家自然科学基金资助项目(3017090 6)
关键词 芬太尼 核因子-ΚB 肿瘤坏死因子 白介素-6 fentanyl nuclear factor kappaB tumor necrosis factors interleukin 6
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参考文献12

  • 1Sheng ZR.Practical Clinical Anesthesiology(实用临床麻醉学)[M].Shenyang: Liaoning Science and Techenology Publishing House,1987.92-96.
  • 2Sueoka E, Sueoka N, Kai Y, Okabe S, Suganuma M,Kanematsu K, et d. Anficancer activity of morpltine andits synthetic derivative, KT-90, mediated through apopoto-sis and inhibition of NF-kB activation[ J]. Biochem Biophys Res Commum, 1998, 252(3) :566 - 570.
  • 3Welters ID, Menzehach A, Goumon Y, Cadet P, Merges T, Hughes TK, et al. Morphine inhibits NF-kB nuclear binding in human neutrophils and monocytes by a nitric oxide-dependent mechanism[J]. Anesthesiology, 2000, 92(6) : 1677 - 1684.
  • 4BoyS, Cain KJ, Chapin RB, Chatboneau RG, Uarke RA.Morphine modulates NF-kappaB activation in macrophages[ J ] . Biochen Biophys Res Commun , 1998, 245(2):392-396.
  • 5Oh WS. Effect d fentanyl on TNF-alpha and IL-lbeta levels during global ischemia/reperfusion in rats[J]. Int J Tissue React, 2002, 24(1):11-21.
  • 6Yoshida S, Noake T, Tanaka Y, Ishibasifi N, Shinmzu Y,Shinmzu K, et al. Effect of fentanyl citrate anesthesia on protein turnover in patients with esophagectomy[ J]. J Surg Res, 1996, 64(2) : 120 - 127.
  • 7Sheng ZR.Practical Clinical Anesthesiology(实用临床麻醉学)[M].Shenyang:Liaoning Science and Techenology Publishing House,1987.92—96.
  • 8Foulds S. Novel flow cytometric method for quantifying nuclear binding d the transcription factor nuclear factor kappa B in unseparated human monocytes and polymorphonuclear cells[J]. Cytometry, 1997, 29(2) : 182 - 186.
  • 9Larsen B, Hoff G, Wilhelm W, Buchinger H, Wanner GA,Bauer M. Effect of intravenous anesthetics on spontaneous and endotoxin-stimulated cytokine response in cultured human whole blood[J]. Anesthesiology, 1998, 89(5):1218- 1227.
  • 10Loop T, Liu Z, Humar M, Hoetzel A, Benzing A, Pahl HL, et al. Thiopental inhibits the activation of nuclear factor kappaB[J]. Anesthesiology, 2002, 96(5): 1202 -1213.

同被引文献13

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  • 2Alimkhodzhaeva PR,Usmanova AS,Gil'dieva BS.Effect of hydrocortisone on spontaneous and mitogen-dependent activity of peripheral blood lymphocytes in some vertebrates.Bull Exp Biol Med,2002,133:471 ~474.
  • 3Moiniche S,Kehlet H,Dahl JB.A qualitative and quantitative systematic review of preemptive analgesia for postoperative pain relief:the role of timing of analgesia.Anesthesiology,2002,96:725 ~741.
  • 4Hogan QH.No Preemptive Analgesia:is that so bad.Anesthesiology,2002,96:526 ~ 527.
  • 5Kissin I.Preemptive Analgesia.Anesthesiology,2000,93:1138 ~1143.
  • 6Katz J.Pre-emptive analgesia:importance of timing.Can J Anesth,2001,48:105 ~ 114.
  • 7Kelly DJ,Ahmad M,Brull SJ.Preemptive analgesia I:physiological pathways and pharmacological modalities.Can J Anaesth,2001,48:1000 ~ 10.
  • 8车润平.术后镇痛与免疫反应[J].国外医学(麻醉学与复苏分册),1999,20(2):80-83. 被引量:36
  • 9郑艳彬,李雪宁.非甾体抗炎药氯诺昔康[J].国外医药(合成药.生化药.制剂分册),2001,22(3):166-169. 被引量:84
  • 10乔萍.神经在免疫稳态和疾病中的调节作用[J].国外医学(免疫学分册),2002,25(6):303-307. 被引量:4

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