比格犬前列腺增生模型中输精管病理变化

Pathology changes of vas deferens in benign prostatic hyperplasia model of beagle dogs

目的 探讨前列腺增生症中输精管的增生机制。方法 丙酸睾酮 (TP)诱导比格犬前列腺增生模型建立后 ,获取输精管组织 ,利用放射免疫分析法检测输精管组织中的双氢睾酮 (DHT)含量 ,组织经石蜡固定包埋切片 ,显微图像分析技术分析输精管管腔大小及上皮细胞高度 ,流式细胞术检测输精管组织凋亡率 ,免疫组织化学技术检测输精管组织中前列腺特异抗原 (PSA)、前列腺酸性磷酸酶 (PAP)、Bcl 2及P5 3蛋白表达 ,2 %的琼脂糖凝胶电泳观察输精管组织的凋亡情况。结果 输精管组织中DHT含量随TP剂量增大而增加 ,但无统计显著性差异。输精管管腔面积随TP增加增大 (P <0 .0 1 ) ,输精管上皮细胞高度也随TP增高 (P <0 .0 1 )。流式细胞术结果中 ,对照组细胞凋亡率高于 0 .8及 7.5mg·kg-1TP组(P <0 .0 1 )。免疫组化结果中 ,PSA ,PAP和Bcl 2表达没有明显差异 ,但各组P5 3表达均低于对照组 (P <0 .0 5 )。琼脂糖凝胶电泳结果中 ,对照组中可以看到 2 0 0bp整数倍的DNA片段 ,其他组则没有观察到类似现象。结论 DHT促进输精管增生 。

AIM The objective of this investigation was to research the proliferation mechanism of vas deferens in benign prostatic hyperplasia. METHODS The vas deferens, from beagle dogs which have been administered testosterone propionate(TP) to build benign prostatic hyperplasia model in vivo, was analyzed by a series of methods. Dihydrotestosterone(DHT) in vas deferens was detected by radioimmunoassay, the slices (4 μm) of vas deferens were stained with HE, and the duct area as well as the height of epithelial cell were measured with videodensitometer. The apoptosis rate was determined by flow cytometry method. The expression of prostatic specific antigen(PSA), prostatic acid phosphatase(PAP), Bcl 2 and P53 were determined by immunohistochemical methods. The nucleosomal DNA fragmentation was monitored by agarose gel electrophoresis. RESULTS DHT level of vas deferens was increased with increasing TP doses, but there was no significant difference between groups. After being administered TP, the duct area of vas deferens was enlarged (P<0.01), and the height of epithelial cell was increased (P<0.01) . The cell apoptosis rate of control was higher than that of 0.8 and 7.5 mg·kg -1 TP groups(P<0.01). While there were no differences in expression of PSA, PAP and Bcl 2 between control and other groups, the expression of P53 of control was higher than that of the other groups(P<0.05). In agarose gel electrophoresis, the 200 bp base pair DNA fragments and the multiples of them were observed in control, while similar phenomenon didn′t find in other groups. CONCLUSION DHT induces vas deferens to proliferate, and the proliferation of vas deferens is related to inhibition of apoptosis.