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碱性果胶裂解酶摇瓶发酵条件的研究 被引量：5

Production conditions of alkaline pectate lyase by alkaline microorganism Bacillus subtilis
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摘要 利用碱性果胶裂解酶生产菌株Bacillus subtilis WSHO2-02进行摇瓶发酵优化,确定了最适种子斜面培养基、种子摇瓶培养基和发酵摇瓶培养基等培养条件,经14h的摇瓶发酵,酶活最高达到8.29U/mL。 Fermentation conditions of alkaline pectate lyase by an alkaline microorganism Bacillus subtilis WSH02 - 02 were optimized. The suitable seed slant, seed culture and fermentation culture were determined. After the cultivation for 14 hours, the alkaline pectate lyase activity was improved as high as 8.29 U/mL.
作者 赵政 董云舟 堵国成 陈坚
机构地区 江南大学工业生物技术教育部重点实验室
出处 《工业微生物》 CAS CSCD 北大核心 2003年第2期9-14,共6页 Industrial Microbiology
基金 国家"863"项目(863-2001AA327090)
关键词 碱性果胶裂解酶 摇瓶发酵 发酵条件 培养基 培养条件 alkaline pectate lyase production conditions Bacillus subtilis
分类号 TQ925 [轻工技术与工程—发酵工程]
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参考文献15

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工业微生物
2003年 第2期

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