高温中性蛋白酶及其产生菌的初步研究

Preliminary studies on the thermostable neutral protease

对高温中性蛋白酶产生菌耐热芽孢杆菌(Thermophilic Bacillus)XJT9503的产酶条件及酶的提取进行了初步研究。耐热芽孢杆菌XJT9503产酶的摇瓶培养基最适碳源为葡萄糖,最适氮源为豆饼粉,100r/min,46℃培养60h酶活最高。实验结果表明高温中性蛋白酶提取可采用硫酸铵分级沉淀法。

Enzyme production conditions and the isolation method of thermophilic Bacillus XJT9503 producing thermostable neutral protease were studied. The results of the experiments indicated that optimum carbon source and nitrogen source were glucose and soybean powder. The higher enzyme activity was obtained under the conditions of 100r/min,46℃ , cultured for 60h. Thermostable neutral protease could be isolated by using ammonium sulphate fractional precipitation.