辐照西洋参培养物皂苷次生代谢调控的研究

STUDY ON THE SECONDARY METABOLIC REGULATION OF CALLUS OF Panax quinquefolium L.

研究了不同前体、前体浓度、前体加入时期、前体作用时间、ATP浓度、烟酸浓度对西洋参芽胞愈伤组织生长及皂苷合成的影响。结果表明 :0 1 %甲羟戊酸对生长有抑制作用 ,对皂苷合成有明显的促进作用 ;1 0 0mg L亮氨酸对生长无明显影响 ,对皂苷合成有促进作用 ;444mg L的醋酸镁对培养物生长和皂苷合成都有促进作用 ;在继代开始时加入前体皂苷含量最高 ;甲羟戊酸作用 6h皂苷产量最高 ;1 60mg LATP对生长和皂苷合成均有促进作用 ;1 0mg L烟酸利于培养物生长和皂苷合成 ;用6 0 Coγ射线辐照培养物 ,剂量为 40 0 0Gy时得到皂苷含量高于原愈伤组织 1倍的细胞系。

The effects of precursors with different kind, concentration, growth phase, time and different ATP, nicotinic acid concentration on the callus growth and saponin formation with the buds of Panax quinquefolium L. has been studied. The results showed that 0 1% meralonic acid inhibited the callus growth but promoted the saponin formation; L leucine of 100mg/L had no obvious effects on callus growth, but promoted the saponin formation; Mg(Ac) 2 of 444mg/L promoted both the callus growth and saponin content. The optimum time for precursor feeding is at the beginning of regeneration. The optimum time for meralonic acid is 6h; ATP of 160mg/L promoted both the callus growth and saponin formation. Nicotinic acid of 1 0mg/L promoted callus growth and saponin formation. With 4000Gy of 60 Co γ rays, the saponin content has been increased 1 time compared with nonirradiation.