氯沙坦和卡维地洛对缺血再灌注心肌细胞凋亡的影响

A comparative study of carvedilol with losartan on the inhibition of cardiomyocyte apoptosis caused by ischemia/reperfusion injury

目的 :研究氯沙坦和卡维地洛对缺血再灌注心肌细胞凋亡的影响 ,比较氯沙坦和卡维地洛对缺血再灌注心肌损伤的保护作用。方法 :结扎Wistar大鼠左冠状动脉前降支 ,建立大鼠缺血再灌注动物模型 ,采用原位末端标记细胞凋亡法检测心肌细胞凋亡 ,并利用光学显微镜进行细胞计数。结果 :单纯缺血 再灌注组心肌细胞凋亡数较假手术组明显增多 (37.5 3± 9.2 2 /视野∶0 .18± 0 .0 9/视野 ,P <0 .0 5 ) ,氯沙坦和卡维地洛组心肌细胞凋亡数分别为 8.74± 3.5 1/视野和 7.6 3± 4 .0 5 /视野 ,较单纯缺血 再灌注组明显减少 (P <0 .0 5 ) ,氯沙坦和卡维地洛两组间无明显区别 (P >0 .0 5 )。结论 :氯沙坦和卡维地洛对缺血再灌注心肌细胞损伤具有相似的保护作用 。

Objective:This study compare the effect of carvedilol with that of losartan on cardiomyocyte apoptosis.Methods:The left anterior descending branch of coronary artery was ligated to establish the ischemia/reperfusion model in Wistar rat; the apoptotic cardiomyocyte is showen by in situ end labeling method and the number of apoptotic cardiomyocyte is counted under optical microscopy.Results:The number of apoptotic cardiomyocyte per visual field is 37.53± 9.22 in operating alone group, 0.18± 0.09 in sham operating group, 7.63± 4.05 in operating and carvedilol treated group, 8.74± 3.51 in operating and losartan treated group. The significant diffesence exists between operating group and operating with carvedilol or losartan treated groups (P< 0.05), but no significance between carvedilol treated group and losartan treated group(P> 0.05).Conclusion:It is concluded that carvedilol has obvious anti-apoptotic effect as same as losartan on cardiomyocyte in ischemia/reperfusion heart.