大鼠海马神经元急性分离法

A method of acute isolation of rat hippocampal neurons

作者设计和使用的海马神经元细胞分离法是一种结合酶消化和机械分离的方法 ,既不损伤神经元的形态 ,又保留了神经元细胞膜的电学特征。形态上有差异的细胞易于分辨。用内面向外式膜片钳和全细胞膜片钳技术证实 ,约 95

A method of acute isolation of rat hippocampus neurons combined enzymatic digestion and mechanical isolation was developed. This method not only keeps the configuration of neurons, it also saves the electrical characters of the cell membrane. It is easy to distinguish the discrepant cells in the configuration. It is confirmed that the electric currents can be observed in about 95% neurons isolated using inside out and whole cell patch clamp.