弓形虫垂直传播小鼠动物模型的建立

Establishing mouse model of vertical transmission of Toxoplasma gondii

目的 探讨乙底酚 (己烯雌酚 ,DES)处理时 ,弓形虫垂直传播小鼠动物模型的建立条件。方法 采用不同数量 (0、2 0 0、4 0 0、80 0、16 0 0、32 0 0 )复苏的弓形虫速殖子 ,于不同妊娠期 (妊娠第 6、8、10天 )接种小鼠 ,并用一定剂量DES处理后 ,于妊娠第 18天处死 ,观察其流产情况及未流产小鼠的胎仔脑组织弓形虫感染情况。结果 妊娠第 6天接种速殖子 ,流产率高 ,垂直传播率低 ;妊娠第 10天接种时 ,流产率低 ,垂直传播率高。流产率和垂直传播率都随着接种速殖子数量的增加而升高。结论 于妊娠第 8天接种复苏后的弓形虫速殖子 4 0 0个 /只建立的弓形虫垂直传播的小鼠动物模型 ,既可以使流产率控制在较低水平 。

Objective To investigate the condition for establishing the mouse model of vertical transmission of T. gondii. with the treatment of diethylstilbestrol(DES). Methods Pregnant mice, infected with different dose(0?200?400?800?1 600?3 200) of T. gondii at different time(days 6,8 and 10 of gestation),After DES subcutaneous injection the mice were killed at day 18 of gestation. Abortive mice were counted. It was identitied whether the embryos in the uteri were infected with T. gondii. Results The mice infected earlier had higher abortive rate and lower rate of vertical transmission, while those infected at a later time had lower abortive rate and higher rate of vertical transmission. Both abortive rate and vertical transmission rate arose with the increasing of T. gondii's quantity. Conclusion After the animal model of vertical transmission of T. gondii . with the treatment of 400 T. gondii. at day 8 of gestation, not only the animal abortive rate can be controlled at a lower level, but also the animal will not all be transmitted.