双等色点光度法测定络合物真实摩尔吸光系数与稳定常数

The Theoretical Study about Spectrophotometric Determination of True Molar Absorptivity and Stability Constant of Complexes by Dual Isosbestic Points

先利用两种实验设计方案,获得了等摩尔系列法与摩尔比法吸收光谱中的两个等色点,再通过另一实验设计方案测定这两个波长下的吸光度并计算出有关物种的真实摩尔吸光系数及平衡组份浓度,进而可计算出络合物的稳定常数.

Based on the two isosbestic points in absorption spectra of equimolar series method and mole ratio method with two experiment modes.The absorbances at wavelength of isosbestic points were determined by another experiment mode,and the molar absorptivity and equilibria conce tration of species can be counted.Then,stability constants of complexes were directly calculated with equilibria concentrations.