蛭丹化瘀口服液对支原体感染小鼠肺炎的影响

Effect of Zhidan Huayu Oral Liquid on Mycoplasmal Pneumonia in Mice

目的:探讨活血化瘀中药对小鼠肺炎支原体肺炎的影响。方法:BALB/C小鼠135只随机分为对照组、支原体肺炎模型组(IF1,IF2)、罗红霉素治疗组(LH1,LH2)和罗红霉素加蛭丹化瘀口服液组(LZ1,LZ2),分别检测鼠肺组织病理学评分,肺组织图像分析和肺血栓计数的变化。结果:经灌喂治疗,在第3天罗红霉素组(LH1)、罗红霉素加蛭丹化瘀口服液组(LZ1)小鼠肺组织病理学评分明显低于模型组(IF1,P<0.01);LH1,LZ1组图像分析值明显高于模型组(IF1,P<0.05);LH1及LZ1组肺部炎症明显轻于IF1组,但两组之间治疗效果差异无显著性。随着疗程的延长,在灌喂治疗的第4天,LZ2鼠肺组织病理学评分明显低于模型组(IF2,P<0.01),图像分析值LZ2组明显高于IF2(P<0.01);LZ2组与LH2组比较,疗效更显著(P<0.05)。在肺炎治疗的第3、4天,LH1,LH2肺组织血栓计数与IF1,IF2之间差异无显著性(P>0.05);LZ1,LZ2组肺组织血栓计数均显著低于IF1,IF2组(P<0.05)。结论:蛭丹化瘀口服液可辅助罗红霉素减轻支原体肺炎小鼠的病情,其作用机制可能与减少血栓形成,改善微循环有关。

Objective:To study the effect of Chinese herbal medicine for activating blood circulation to remove stasis in treating mycoplasmal pneumonia (MP) in mice. Methods:One hundered and thirty-five BALB/C mice were randomly divided into the control group, the MP model groups IF1 and IF2, the Rexithromycin treated groups LH1 and LH2, and the Rexithromycin plus Zhidan Huayu oral liquid treated groups LZ1 and LZ2. The changes of pathologic scoring, graphic analysis and thrombus counting of lung were observed. Results: In the 3rd day of treatment, the pathologic scores in LH1 and LZ1 were significantly lower and their values of graphic analysis were significantly higher than those in IF1 (P<0.01 and P<0.05 respectively), but with inflammation of lung significantly milder than that in IF1. The difference of therapeutic effect between LH1 and LZ1 was insignificant. In the 4th day of treatment, pathologic scores in LZ2 was significantly lower and value of graphic analysis higher than those in IF2 respectively (P<0.01), with the improvement better than that of LH2 (P< 0.05). In 3rd and 4th day of treatment, the difference of thrombus counting between the Rexithromycin treated groups and the model groups was insignificant (P > 0.05), but it was significantly lower in the combined treated groups than that in the model groups (P < 0.05). Conclusion: Zhidan Huayu oral liquid could assist Rexithromycin to alleviate the condition of mice with MP, its mechanism may be related with the effect of reducing thrombosis and improving microcirculation.