肌生注射液心脏保护及抗氧化作用的实验研究

Experimental Study on Cardiac Protection and Anti-oxidation Effects of Jisheng Injection

目的:观察肌生(GL)注射液的心脏保护效果。方法:在改良的Euro-Collins心脏保存液(mEC液)中添加肌生注射液,以单纯mEC液为对照组,保存大鼠离体心脏20h,采用Langendorff离体鼠心灌注法,于再灌注40min,测定心功能、冠脉流量及心肌含水量。检测冠脉流液中乳酸脱氢酶(LDH)、肌酸激酶(CK)活性,心肌组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及心肌组织的病理学改变。结果:保存后GL组心脏心功能和冠脉流量明显优于mEC组(P<0.01);心肌含水量低于mEC组,但无统计学意义;LDH、CK活性明显低于mEC组(P<0.01);SOD活性显著高于mEC组(P<0.05),MDA含量显著低于mEC组(P<0.05)。病理损伤较mEC组轻。结论:肌生注射液具有很好的心脏保护及抗氧化作用。

Objective: To observe the effect of Jisheng injection (JSI) in protecting heart. Methods: Isolated heart of rat was preserved in modified Euro-Collins solution (mEC) containing JSI for 20 hrs, and that preserved in simple mEC was taken as control. Then Langendorff isolated rat heart perfusion was conducted. Forty minutes after perfusion, the cardiac function, coronary flow, myocardial water content were determined, and lactate dehydro-genase (LDH), creatine kinase (CK) activity in perfusate, superoxide dismutase (SOD) activity, malondialde-hyde (MDA) content in myocardial tissue and pathologic change in myocardium were also observed. Results: The cardiac function and coronary flow of isolated heart preserved in JSI containing mEC was significantly better than those in the control ( P < 0.01), with the LDH, CK activity and MDA content significantly lower (P < 0.01 and P<0.05), SOD activity significantly higher (P<0.05) and pathologic injury milder than those in control, but comparison of cardiac water content between the two groups showed insignificant difference. Conclusion: JSI has good cardiac protective and anti-oxidizing effects.