(His)_6-Arg-Arg-人胰岛素原在大肠杆菌中的高效表达(英文)

Enhanced Expression of (His)_6-Arg-Arg-human Proinsulin in Escherichia coli

为了提高胰岛素原的表达量 ,用pQE 40质粒构建了 (His) 6 Arg Arg 人胰岛素原 [(His) 6 Arg Arg humanProinsulin ,RRhPI]的大肠杆菌表达系统。通过培养条件的优化 ,在摇瓶培养的条件下 ,目标产物RRhPI以包涵体形式获得了高效表达 ,每升培养基收获湿菌体约 2 7g ,包涵体 6g(干重 1 .8g) ,RRhPI约5 4 0mg。该水平已超过现有文献报道的摇瓶培养的最高水平。

To enhance the expression level of human proinsulin, Escherichia coli (E.coli) M15 harbouring a plasmid (His) 6 Arg Arg human proinsulin (RRhPI)/pQE 40 for human proinsulin production was cultured using flask. As a result, high expression level of the gene product, which accumulated as inclusion bodies, was obtained. 27g wet E.coli cells were harvested from 1L culture medium, and 6g inclusion body also. The harvest of our designed gene product RRhPI was about 540mg/L culture medium.