营养及环境因子对农杆菌诱导的长春花发根生长和生物碱生成的影响

Effects of Nutrition and Environmental Factors on Growth and Alkaloid Production of Catharanthus Roseus Hairy-Roots

将A4 发根农杆菌感染长春花 (Catharanthusroseus(L .)G .Don)后所长出的发根 (hairy root)进行培养 ,比较不同营养成分、碳源及初始糖浓度、外源激素以及温度和摇床转速对发根生长及产碱的影响。结果表明 ,当培养基为 1 2B5培养基时发根生长良好 ;一定范围内初始糖浓度增加 ,有利于发根生长和生物碱生成 ;加入 5 0 0mg L的L 色氨酸利于发根的生长和产碱 ;将发根在 2 8℃、75r min的条件下悬浮培养

The hairy roots induced by Agrobacterium rhizogenes A 4 were cultured and the effects of different nutrition elements, carbon sources and primary sugar density, hormone as well as temperature and shaker speed were compared. The results showed that when cultured in 1/2B 5 culture with high concentration of initial sucroseand 1mg/L 6 BA and 500mg/L L tryptophan in 28℃and 75r/min, hairy roots grew well and alkaloid production was high.