大叶冬青无根试管苗移栽技术研究

Studies on the transplanting techniques of Ilex latifolia Thunb.seedling in vitro

选苗高3cm以上大叶冬青(IlexlatifoliaThunb.)健壮无根试管苗进行移栽试验,结果表明基质为上下层放置型(即先将松林表土3份放入容器袋中,再放河沙1份于土上),温度控制在(26±2)℃,湿度为95%以上,移栽后,用2 5～3 5mg/L的D号生根剂(NAA+IBA)处理小苗3次,促根处理效果较好,移栽苗成活率达79 6%。

By selecting longer than 3 cm of Ilex latifolia Thunb.rootless tubeshoots (RTS),with the optimum growth temperature of (26 ±2) ℃ and humidity of 95% above,the treatments were conducted of both dipping in rootinducing regulators (RIR) before transplant and watering RIR different times after transplant.Different trial results showed watering RTS with the optimum RIR of NAA (2.5 mg/L) and IBA (2.5 mg/L) three times (one time each week) after transplant favored a highest surrival (79.6%).And the tranplanting medium with one part of sand (upper layer) and three parts of forest earth (lower layer) met a pleasing survival nearly 80%.