艾蒿提取物及其不同柱层析馏分对甜菜夜蛾产卵行为的影响

Oviposition Response of Spodoptera exigue to the Extract and Four Fractions in Different Solvents from Artemisia argyi Levl.

利用 Syntech触角电位仪 ,测定了甜菜夜蛾雌成虫对艾蒿提取物和 4种溶剂 (苯、乙酸乙酯、丙酮和乙醇 )柱层析馏分的触角反应 ;利用涂纸法 ,测定了艾蒿提取物和 4种馏分对甜菜夜蛾产卵的忌避特性和抑制特性。结果表明 :甜菜夜蛾雌成虫对相同浓度 (10 mg/ m L)提取物和馏分的触角反应存在很大差异 ,对丙酮馏分的反应最强烈 ,电位值为 9.632± 1.0 5 4 m V,其次为乙酸乙酯和苯馏分 ,以乙醇馏分的反应最弱。提取物和不同馏分对甜菜夜蛾的产卵忌避作用存在很大差异 ,丙酮馏分、乙酸乙酯馏分和提取物都具有一定的忌避作用 ,而且随浓度上升而增强 ,其中以丙酮馏分的忌避作用最强 ,10 mg/ m L的忌避率达到 4 9.0 %。不同浓度处理时 ,都以丙酮馏分和乙酸乙酯馏分的产卵抑制作用最强 ,且随浓度上升而增强 ,10 mg/ m L的抑制率分别达到 72 .16%和 68.95 % ;较高浓度 (5 mg/ m L和 10 mg/ m L)时 ,提取物。

The antennae response of female adults of Spodoptera exigua to the crude extract and 4 fractions in different solvents (benzene, ethyl acetate, acetone and ethanol) from Artemisia argyi was investigated by using Syntech electroantenogram apparatus, and the repellent and deterrent characteristics of the crude extract and 4 fractions to S. exigua were measured as well. The results showed that there was significant difference among the crude extract and 4 fractions in the antennae response, with the highest electroantenogram (9.632±1.054 mV) in the fraction in acetone, followed by the fraction in ethyl acetate and the crude extract, and the electroantenogram in the fraction in ethanol was lowest (3.814±0.441 mV). Both the fraction in acetone and in ethyl acetate had significant repellence and deterrence to the oviposition of S. exigua, and the repellent rate and deterrent rate were increased along with the concentrations of the fractions. At 10 mg/mL, the repellent rates of the fraction in acetone and in ethyl acetate were 34.9% and 49.0%, and the deterrent rates of them were 72.16% and 68.95% respectively. At higher concentrations (5 mg/mL and 10 mg/mL), the crude extract, the fraction in benzene and in ethanol also had the deterrence to the oviposition to different extents.