摘要
为探讨印楝素作为鱼用杀寄生虫药物开发的可能性 ,选用 ISO推荐的斑马鱼 Brochydainorerio为试材研究了印楝素对鱼的急性毒性。印楝素对斑马鱼 4 8h和 96h的 L C50 值分别为 4 1.9和2 3.3mg/L ,属中低毒鱼药。采用安全系数 0 .1,印楝素对鱼的安全浓度为≤ 2 .3mg/L。采用鲤鱼Cyprinus carp io L inn.鱼种为试材 ,研究了印楝素对鲤鱼外周血细胞微核产生率的影响。试验表明印楝素不会诱导鲤鱼外周血细胞染色体突变产生微核 ,可认为其对养殖鱼类没有致突变性。在研究了印楝素对鱼类安全性的基础上 ,将其应用于鱼类寄生虫病——车轮虫病 Trichodina sp.的防治 ,在鱼的安全浓度范围内 ,印楝素对车轮虫具有良好的杀灭作用 ,且呈一定的剂量 -效应关系 。
The toxic effects of azadirachtin on fishes were studied. The results showed that azadirachtin had lower acute toxicity to Brochydaino rerio. The LC 50 of Brochydaino rerio by azadirachtin after tested 48 h and 96 h was 41.9 and 23.3 mg/L,respectively. The safe concentration to Brochydaino rerio was 2.3 mg/L. The micronucleus test of carp with azadirachtin indicated that azadirachtin had no mutation activity to the fishes, so azadirachtin could be considered non-mutagen. At the same time, azadirachtin was used in control the parasitic diseases on fishes which caused by Trichodina sp.. During the safe concentration to fishes, the better effects on the controlling of parasitic diseases were achieved and there was a relationship between the efficacy and the concentration of azadirachtin. The results also showed that azadirachtin had a good prospect to be used as an agent for controlling the parasitic diseases of fishes.
出处
《农药学学报》
CAS
CSCD
2003年第2期85-89,共5页
Chinese Journal of Pesticide Science
关键词
印楝素
鱼类
毒理效应
寄生虫痛
azadirachtin
fishes
toxic effect
parasitic diseases
