U69 593对家兔心室肌细胞L型Ca^(2+)电流的影响

Effect of U69 593 on L-type calcium current in isolated rabbit ventricular myocytes

目的 :研究 U695 93对家兔心室肌细胞 L 型 Ca2 +电流 ( ICa· L)的影响。方法 :采用全细胞膜片钳技术记录家兔心室肌细胞 IC a· L。结果 :在 -2 0～ +4 0 m V测试电压范围内 ,10 0 nmol/ L U695 93显著增加 IC a· L,以 +10 m V为最大激活电压 ,其峰值 ICa· L由加药前 -7.1± 0 .4p A/ p F增至 -8.3± 0 .5 p A/ p F ( P<0 .0 1,n=8)。 10 0 nmol/ LU 695 93不影响 IC a· L电压依赖性激活与失活特性。结论 :U695 93可增加家兔心室肌细胞 ICa· L。

AIM: To investigate the effect of U69 593 on the L type calcium current (I Ca·L ) in isolated rabbit ventricular myocytes. METHODS: I Ca·L was recorded by using the whole cell voltage clamp technique. RESULTS: U69 593 at 100 nmol/L significantly increased I Ca·L at the test potential from -20 mV to +40 mV. The peak of I Ca·L was increased from -7.1±0.4 pA/pF to -8.3±0.5 pA/pF with maximal activation potential at +10 mV (by 18.3%±3.5%, P <0.01 vs control, n =8). The steady state activation and inactivation of I Ca·L was not affected by 100 nmol/L U69 593. CONCLUSION: The present study demonstrated that U69 593 increased I Ca·L in isolated rabbit ventricular myocytes.