摘要
观察了分泌抗IBDV单抗杂交瘤细胞在改良型5L Celligen细胞反应器中采用半连续培养方式的生长和代谢情况,研究了在该工艺过程中的葡萄糖消耗、单抗的生成、乳酸和氨的产生。同时为培养方案和控制策略进一步的改良确定方向。
The growth and metabolism of JAL1 hybridoma cells were studied in semicontinuous cultures in improved Celligen bio-reactor (5L).The utilization of glucose and the production of antibody,lactic acid and ammonia were also investigated.Meanwhile,the discussion of operation mode and control strategy in bioreactor culture indicates the direction of their improvement.
出处
《上海师范大学学报（自然科学版）》
2003年第2期64-67,共4页
Journal of Shanghai Normal University(Natural Sciences)
基金
上海市教委基金(98E05)
关键词
IBDV
杂交瘤
半连续培养
IBDV
hybridoma cell
semicontinuous culture.
