推拉动作对大鼠脑组织NO含量及DNA分布图的影响

Effect of Push-pull Maneuver on Distribution of DNA and NO Content in Rats′Brain Tissue.

目的探讨推拉动作对大鼠脑组织中NO含量及细胞凋亡的影响。方法 90只雄性Wistar大鼠随机分成对照组、+Gz暴露组、推拉组 ,在 +Gz暴露后 30min、3h、1 2h、2 4h和 48h 5个时间点处死大鼠取脑。用硝酸还原酶化学比色法检测NO含量的变化。并用流式细胞仪检测皮层和海马细胞DNA分布图 ,测定A0峰所占的百分比 ,表示细胞凋亡程度。结果脑组织一氧化氮含量 ,在 30min、3h和 1 2h时 ,+Gz暴露组和推拉组与对照组比较有显著差异 ,推拉组与 +Gz暴露组相比较也有差异性 ,2 4h和 48h组间比较无显著性差异。皮层、海马细胞在 3h、1 2h和 2 4h时两实验组与对照组相比较细胞凋亡百分比明显增多 ,2 4h达高峰 ,48h无显著性差异。推拉组与 +Gz暴露组细胞凋亡百分比在 3h和 2 4h有差异 ,1 2h差异显著。结论高 +Gz及推拉动作可引起大鼠脑组织NO含量和细胞凋亡百分比增加 ;推拉组比单纯 +Gz暴露所造成的NO含量及脑细胞凋亡百分比多 ,但这种对神经元的损害是可逆性的。

Objective To study the effect of push pull maneuver on cerebral apoptosis and NO content of rats. Method Ninety male Wistar rats were randomly divided into the control group, +Gz exposure group and the push pull maneuver group . The rats were killed after 30 min exposure,3 h,12 h,24 h or 48 h exposure, and the brain tissues were taken.The varivation of NO content measured was using chemical colormetry.Then flow cytometric method was adopted to examine the distribution diagram of cortex and hippocampus cell DNA for determination of percentage of A0 peak , which could express apoptosis degree. Result NO content in +Gz exposure group and push pull group are significantly different from that of control at 30 min,3 h and 12 h. And the difference between +Gz group and push pull group are also significant, but not at 24 h and 48 h. Comparing with the control group, the apoptosis amount of cortex and hippocampus cell of +Gz exposure group and push pull maneuver group increased obviously at 3 h,12 h and 24 h, reached its peak at 24 h, and had no remarkable difference at 48 h.The comparison of the apoptosis amount of cortex cell and hippocampus cell between push pull maneuver group and +Gz exposure group, difference at 3 h and 24 h and remarkeably different at 12 h.Conclusion High +Gz and push pull maneuver may increase the percentage of cerebral apoptosis amount and NO content. Comparing with the +Gz exposure group, the push pull maneuver group will cause more increase in apoptosis amount and NO content, but this damage is reversible.