高血糖对大鼠视网膜蛋白激酶C的影响

Effect of Hyperglycemia on Protein Kinase C in Retina of diabetic rats

目的 :观察高血糖对STZ诱发糖尿病大鼠视网膜蛋白激酶C(ProteinkinaseC ,PKC)表达的影响。方法 :将 6 0只大鼠分为对照组 1 0只和观察组 5 0只。观察组用STZ造模。 9个月时将大鼠处死 ,通过免疫组织化学法观察两组大鼠视网膜PKC的表达。结果 :观察组PKC蛋白表达增加 ,与对照组比较差异有意义 (P <0 .0 1 )。结论

Objective:To investigate the effects of hyperglycemia on protein kinase C(PKC) in retina of diabetic rats.Methods:Sixty SD rats were divided into control and experimental groups.Fifty rats models were made by Streptozotocin injection.Ten rats were used as healthy group.After nine months the rats were sacrificed and the eyes were excavated.The retinas were studied by means of immunohistochemical method,light microscope.Results:The stronger expression of PKC was show in retina of diabetic rats than in normal rats(P<0.01).Conclusion:The outcome implied that hyperglycemia might increase PKC in experimental diabetic rats