肺炎克雷伯菌表面成分对细胞因子的影响

The effects of klebsiella pneumonia cell surface preparations on secretion of cytokines

目的 :了解肺炎克雷伯菌表面成分诱生细胞因子的作用。方法 :不同浓度的Kp 表面成分刺激小鼠脾细胞诱生细胞因子并用细胞增殖试验和细胞毒法测定其活性。结果 :2.5、5、10、20、40μg/ml的Kp表面成分均可刺激小鼠脾细胞分泌IL -2、IL -6、TNFα ,高浓度组 (20、40μg/ml)可单独刺激IL -6、TNFα的产生 ,具有类似LPS作用。结论 :Kp表面成分可提高机体免疫功能 。

Objective: To investigate the effect of Klebsiella pneumoniae cell surface preparations on immunity.Methods:Different doses of Kp cell surface preparations stimulated spleen cells of mice to produce cytokines .Then the activity of cytokines were measured with cell proliferation method and cytotoxicity assay.Results:Different doses of Kp cell surface preparations enhanced the secretion of cytokines and high doses (20,40 μg/ml)induced separately the production of IL-6 and TNFα.Conclusion: Kp cell surface preparations made the positive effect on the immunity. As a non-specific immunomodulator,Kp cell surface preparations will be extensively used in both of prevention and treatment of infective diseases.