摘要
本文应用45例5—38周龄人胚胎和1例成人胰腺研究人胰腺发生。材料用Bouin固定,用EH、Gomori醛品红和SPA—HRP免疫组织化学法染色,并用立体计量术做定量分析。实验表明,初级胰岛第3个月出现,第6—7个月大部分退化;次级胰岛从第4个月开始出现。第7个月胰腺做为同种异体移植供体较理想。
Fourty -- five pancreases of the human embryoes and fetuses ranging from 5 -- 38W of age and a pancreas of adult were used to study the development of the human pancreas. All pancreases were fixed in Bouin, stained with HE, gomori aldehyde fuchsin and SPA -- HRP immunocytochemistry stain, The stereology quantitation was adopted to quantative analysis. The experiment proved that the primary islands seemed to appear by 3 month and degenerat- ed for most part by 6 -- 7 month, the secondary from the fouth month appeared. The pancres of 7th month was ideal as donor of homotransplation of pancreas.
关键词
组织发生
胚胎
胰腺
primary island
secondary island
stereology quantitation
SPA-HRP immunohistochemistry stain