严重急性呼吸综合征患者血清中SARS相关抗体的特异性研究

Anti-lung tissue antibodies in SARS patient:The potential role of allergic reaction in pathologic development of SARS

目的 :检测严重急性呼吸综合征 (SARS)患者血清中 SARS相关抗体的抗原特异性。 方法 :采用酶联免疫吸附试验(EL ISA)检测血清中 Ig G抗体 ,并采用抗原抗体的预孵育 ,吸附血清中抗原特异性抗体 ,检测其抗原特异性。 结果 :SARS患者血清与 SARS患者肺组织以及非 SARS患者肺组织均有相似的反应性。阳性血清与非 SARS患者肺组织孵育后 ,其中抗SARS患者肺组织抗体被吸收 ,吸收率为 10 0 %。 结论 :本组 SARS患者血清中存在抗肺组织抗体 ,其对

Objective:To analyze the antigenic specificity of the antibodies in the sera of SARS patients.Methods:An indirect enzyme linked immunosorbent assay (ELISA) was used to detect antibodies in the sera of SARS patients,and the positive sera were pre absorbed by the non SARS lung tissue to detect its specificity.Results:There was same reaction to sera of SARS patients concerning SARS and non SARS lung tissue. After absorption all positive sera turned negative and the absorption rate was 100%. Conclusion:The antibodies in sera of SARS patients are mainly against lung tissue.