p21、IGF-Ⅱ在肝细胞癌和肝硬化组织中的表达水平及其与肝细胞不典型增生的关系

SIGNIFICANCE AND EXPRESSION OF RAS ONCOGENE p21 PRODUCT AND IGF-Ⅱ IN LIVER CIRRHOSIS

目的 :探讨 p2 1、胰岛素样生长因子 (IGF- )在肝硬化、肝细胞癌组织中表达的意义及其与肝细胞不典型增生 (L CD)的关系。方法 :用免疫组化 S- P法检测单纯肝硬化、癌旁肝硬化及肝细胞癌 (HCC) p2 1、IGF- 表达情况。结果 :癌旁肝硬化、单纯肝硬化、癌组织 p2 1的阳性表达率分别为 92 .86 %、6 8%、5 4 .5 5 % ;癌旁肝硬化、单纯肝硬化、癌组织 IGF- 的阳性表达率分别为 75 %、74 %、36 .36 %。肝硬化与 HCC之间差异有统计学意义 (P<0 .0 5 ) ;IGF- 与 p2 1阳性表达呈正相关关系 ;HB-s Ag阳性或伴 L CD改变的肝硬化 ,p2 1、IGF- 阳性率均高于 HBs Ag阴性或不伴有 L CD改变的肝硬化 (P<0 .0 5 )。结论 :1在肝细胞癌变演化过程中 p2 1、IGF- 可能发挥协同作用 ;2 L CD是一群具有肿瘤增殖潜能的癌前细胞群 ,尤其有 p2 1、IGF- 共同过度表达者 ,有发生

Objective:To probe the role of ras oncogene p21 product and IGF Ⅱ in the development of cirrhotic nodules and hepatocellular carcinoma(HCC).Methods:S P immunohistochemical technique was used to analyze the expression of p21 and IGF Ⅱ was studied in 50 cases of liver cirrhosis without HCC, 28 cases of paracancerous liver cirrhotic tissues, and 22 HCC cases.Result:The positive rates for p21 and IGF Ⅱ in cirrhosis with or without HCC were significantly higher than HCC(P<0.05).The positive rates for p21 and IGF Ⅱ in cirrhotic nodules with LCD were significantly higher than those without LCD (P<0.05). The incidence of p21 expression in hepatocytes was significantly higher in IGF Ⅱ positive cases than in negative ones (P<0.01). Conclusion:①the overexpression of p21 and IGF Ⅱ may play a synergetic role in the early stage of the development of HCC ; ②LCD are precancerous cells which have the ability to initiate neoplastic growth and present a high risk of HCC.