肺炎链球菌粘附气道上皮细胞影响因素的研究

Factors Involved in Adherence of Streptococcus Pneumoniae to Airway Epithelial Cells

目的 探讨影响肺炎链球菌粘附气道上皮细胞的因素。方法 肿瘤坏死因子 α(TNF α)及白细胞介素 1β(1L 1β)活化人支气管上皮细胞后 ,与肺炎链球菌 3型孵化 ,用革蓝染色后于显微镜下作细菌计数以研究肺炎链球菌的粘附能力。一氧化氮 (NO)终末产物硝酸盐及亚硝酸盐浓度的测定采用改良Griess法。结果 TNF α和 1L 1β明显增加气道上皮细胞NO的产生。时间曲线研究发现TNF α和IL 1β刺激气道上皮细胞产生NO的作用随时间增加而增强 ,在 16小时达高峰。TNF α及 1L 1β均增加肺炎链球菌粘附于支气管上皮细胞。 结论 TNF α和IL 1β诱导气道上皮细胞的NO产生 。

Objective To study the effect of cytokine(IL-1β and TNF-α) on NO production of airway epithelial cells and the adherence of Spn to airway epitheial cells.Methods Human bronchial epithelial cells line(BEAS-2B) treated with and without IL-1β or TNF-α were studied.Cytokine-activated cells were incubated with Streptococcus pneumoniae.The cells and bactevia were stained with Giemsa Stain.Nitrite and nitrate were assayed with colorimetric assay Kit (Cayman Chemical).Results Nitrite and nitrate were significantly higher in cultured human bronchial epithelial cells with IL-1β and TNF-α than control(P<0.001).Cultured human bronchial epithelial cells with IL-1β or TNF-α resulted in a time-dependent and dosage-dependent increase in nitrite and nitrate.TNF-α and IL-1β increase the adherence of Spn to the bronchial epithelial cells.Conclusion TNF-α and IL-1β induced NO production of bronchial epithelial cells and enhanced Streptococcus pneumoniae adherence to bronchial epithelial cells.