粒细胞-巨噬细胞集落刺激因子对前列腺癌细胞PC-3M端粒酶活性的影响

Possible Association of Telomerase Activity with Granulocyte-Macrophage Colony Stimulating Factor in Human Prostate Cancer Cell Line PC-3M

目的 研究粒细胞 巨噬细胞集落刺激因子 (GM CSF)对前列腺癌PC 3M细胞株端粒酶活性的影响。方法 以不同浓度的GM CSF作用于PC 3M细胞 ,采用TelomerasePCRELISA方法定性和定量检测端粒酶活性改变。结果 与空白组相比 ,10、 2 0、4 0和 10 0ng/ml的GM CSF能使PC 3M细胞的端粒酶活性分别增高 0 18、 1 2 5、 2 89和 6 4 2倍。结论 GM CSF能够显著增强PC 3M细胞端粒酶的活性 ,GM

Objective To study the possible association between granulocyte-macrophage colony stimulating factor (GM-CSF) and the telomerase activity of human prostate cancer cell line PC-3M. Methods PC-3M cell line was cultured with GM-CSF in the concentrations of 10 ng/ml, 20 ng/ml, 40 ng/ml and 100 ng/ml, respectively. Telomerase activity was measured qualitatively and quantitatively by telomerase PCR ELISA method. Results The telomerase activity was elevated in PC-3M when incubated with GM-CSF in a concentration-dependent manner. The relative telomerase activity was increased by 0.18, 1.25, 2.89 and 6.42 times by GM-CSF of 10 ng/ml, 20 ng/ml, 40 ng/ml and 100 ng/ml correspondingly. Conclusion The telomerase activity could be elevated by GM-CSF, which might be one of the proliferation mechanism of advanced prostate cancer cells.