不同治疗方法对动物慢性细菌性前列腺炎前列腺中TGF-β_1胶原Ⅰ和ⅢmRNA表达的影响

The experimental study of different way on treating chronic bacterial prostatitis of animals

目的 :通过动物试验以口服氧氟沙星、口服氧氟沙星和α1 受体阻滞剂特拉唑嗪及前列腺内注射氧氟沙星和地塞米松 3种治疗方法治疗慢性细菌性前列腺炎 (CBP) ,判定前列腺穿刺注射药物治疗方法对局部组织胶原代谢的影响 ,探讨局部穿刺后前列腺是否有瘢痕化倾向。方法 :①选用健康雄性大白兔 ,依文献方法 ,通过后尿道反复灌注大肠埃希菌制备CBP动物模型 ;②分别予CBP组动物行药物治疗 ,随机分为 1个对照组和 3个治疗组 ,治疗组分别予口服氧氟沙星 ,口服氧氟沙星和特拉唑嗪 ,在B超引导下行前列腺局部穿刺注射氧氟沙星和地塞米松 ,同时以注射生理盐水作为注射对照组 ;③应用半定量RT PCR方法判定经不同治疗方法前列腺组织中TGF β1 、原胶原I、原胶原Ⅲ的mRNA量的变化 ,推断不同治疗方法对胶原代谢的影响 ,判定前列腺穿刺注药是否有致前列腺瘢痕化的危险。结果 :半定量RT PCR分析结果显示 3种治疗方法均可抑制CBP前列腺组织中TGF β1 、原胶原ⅠmRNA的表达 ,对原胶原ⅢmRNA的影响差异无显著性意义 ,而穿刺注药治疗并未显示有明显致瘢痕的副作用。结论

Purpose:(1)To study the effect on chronic bacterial prostatitis of three difference treatment: oral ofloxacin, oral ofloxacin and α 1 blocker: terazosin, intraprostatic injection by ofloxacin and dexamethasone. (2)To investigate the change of collagen metabolizm after intraprostatic injection in local issue and study whether the scar was formed or not in prostatic issue because of intraprostatic injection.Methods:(1)The health male rabbits were selected and formed the aninmal model of chronic bacteial prostatitis by puring E coli into posturetheral according to document. And they had been divided into 5 groups randomly: no treaeted group, oral ofloxacin group, oral ofloxacin and terazosin group, intraprostatic injection by ofloxacin and dexamethason group, and sham intraprostatic injection group, intraprostatic injection was introduced by B ultrasound. (2)Semi quantiative RT PCR was performed to observe the change of TGF β 1 mRNA?collagen ⅠmRNA, and collagenⅢ mRNA before and after treatment.Results:mRNA of TGF β 1 and collagen I in prostate of rabbits with chronic bacterial prostatitis were reduced after treatment but were rised compared with normal. There were no significant difference on collagen Ⅲ mRNA among three therapntic ways. But were rised compared with normal.Conclusions:There were no evidence that intraprostatic injection could promote scar forming.