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组织芯片技术的发展及应用 被引量：4

The development and application of tissue chip
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摘要 组织芯片技术为进行多种肿瘤的多指标 (DNA、mRNA、蛋白指标 )高通量原位分析提供了时间、空间和资源的可行性并最大程度上保证实验条件的一致 ,可广泛应用于肿瘤候选基因及其临床特性的确定、肿瘤预后的判断、肿瘤免疫治疗效果的评价以及肿瘤的分子诊断、肿瘤治疗靶点的筛选 ,显著加速基因组学和蛋白组学成果在肿瘤临床中的应用。 Tissue chip(tissue microarrays,TMAs)provide a powerful approach to high-volume simultaneous analysis of molecular targets at the DNA,mRNA and protein levels under identical,standardized conditions,and also provide maximal preservation and utilization of limited and irreplaceable archival tissue samples.TMAs were applied to identify new candidate genes and their clinical impact in large series of human tumors,to evaluate prognostic and the effect of immunotherapy in cancer,to identify new molecule diagnostic markers or immunotherapeutic targets,to accelerate applying the outcome of genomics and proteomics in clinical oncology.
作者 杨军 苏宝山 王康敏 司履生 王一理
机构地区 西安交通大学第二医院病理科 西安交通大学生命学院
出处 《中国实验诊断学》 2003年第3期195-198,共4页 Chinese Journal of Laboratory Diagnosis
基金 陕西省科技攻关项目 (编号 :2 0 0 1K10 G2 ) 西安市科技研究发展计划项目 (编号 :KG2 0 0 1 0 1)
关键词 组织芯片技术 生物芯片 肿瘤 预后 免疫治疗 分子诊断 治疗靶点 Tissue chip(Tissue microarrays, TMAs) biochip
分类号 R346 [医药卫生—基础医学]
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参考文献34

  • 1杨军,王康敏,苏宝山,黄莺,赵世平.应用组织芯片和ISH技术检测人体多种肿瘤中hTER mRNA和hTERT mRNA的表达[J].西安医科大学学报,2002,23(3):236-238. 被引量：7
  • 2Hsu FD,Nielsen TO,Alkushi A,et al. Tissue microarrays are an effective quality assurance tool for diagnostic immanohistochemisty [ J ]. Mod Pathol, 2002,15(12) : 1374.
  • 3Gillett CE, Springall R J, Barnes DM, et al. Multiple tissue core arrays in histopathology researh: avalidation study [ J ]. J Pathol, 2000, 192 (4) :549.
  • 4Andersen Cl, Monni O, Wagner U, et al. High - throughput copy number analysis of 17q23 in 3520 tissue specimens by fluoresomce in situ hybridization to tissue microarrays[ J ]. Am J Pathol, 2002,161 ( 1 ) : 73.
  • 5simon R, Sauter G. Tissue mieroarrays for miniaturized high- throughput molecular profiling of tumors [J]. Exp Hematol, 2002,30(12) : 1365.
  • 6Bubendorf L. High - throughput mieroarray technologies: from tenomics to clinics[ J]. Eur Urol,2001.40(2) :231.
  • 7Fuller CE,Wang H.Zhang W,et al. High - throughput molecular of high-grade astrocytomas:the utility of fluorescence in situhyhridization on tissue microarrays(TMA-FISH)[ J ].J Neuropathol Exp Neural. 2002.61 (12) : 1078.
  • 8Kallioniemi OP. Biochip technologies in caner research [J]. Annals of medicine. 2001,33(2) : 142.
  • 9Gancberg D, Di Leo A, Rouas G, et al, Reliability of the tissue microarray based HSH for evaluation of the HER - 2 oncogeae in breast carcinoma [J]. J Clin Pathol, 2002,55 ( 4 ) : 315.
  • 10Hedvat CV. Hegde A. Chaganti R.S, et al. Application of tissue microarray technology to the study of non- Hodgkin's and Hodgkin's lymphoma[J]. Hum Pathol,2002,33(10) :968.

二级参考文献6

  • 1[1]Kononen J, Bubendorf L, Kallioniemi A,et al. Tissue microarrays for high throughput molecular profiling of tumor specimen [J]. Nat Med, 1998,4(7):844-847.
  • 2[2]Belair CD, Yeager TR, Lopez PM, et al. Telomerase activity a biomarker of cell proliferation, not malignant transformation [J]. Proc Natl Acad Sci USA, 1997,94(25):13677-13682.
  • 3[3]Kenji N, Elizabeth W, James K,et al. Telomerase activity and expression of telomerase ENA component and telomerase catalytic subunit gene in cervical cancer [J]. AM J Pathol, 1998,153(3):857-864.
  • 4[4]Kazuo Y, Raheeia A, Jan N, et al. Telomerase activity and expression of its RNA component in cervical lesions [J]. Cancer,1998,82(7):1319-1327.
  • 5[5]Schraml P, Kononen J, BubendorfL, et al. Tissuemicroarrays for gene amplification surveys in many different tumor types [J]. Clin Cancer Res,1999,5(8):1966-1975.
  • 6[6]Richter J, Wagner U, Kononen J,et al. High-throughput tissue microarray analysis of cyclin E gene amplification and overexpression in urinary bladder cancer[J]. Am J Pathol,2000,157(3):787-794.

共引文献6

同被引文献57

  • 1孙燕,孙保存.微阵列-比较基因组杂交技术及其在肿瘤学中的应用[J].临床与实验病理学杂志,2004,20(3):358-361. 被引量：3
  • 2吴斌,黄信勇,王米渠,李常度.运用基因芯片研究骨关节炎虚寒证的基因表达谱述要[J].中医药学刊,2004,22(11):2008-2010. 被引量：12
  • 3Kenta O, Tomoko F, Kei H, et al The Development of a Cell Array and Its Combination with Laser - Scanning Cytometry Ailows a High Throughput Analysis of Nuclear DNA Content[JJ. American. Journal Of Pathology - 2000, 157 (3). 723 -728.
  • 4Zhu H, Bilgin M, Bangham R, et al, Global Analysis of Protein Activities Using Proteome Chips [J]. science 2001,293 :2101 - 2105.
  • 5Waring J F, Joily R A, Ciurlionis R, et al. Clustering of hepatotoxins based on mechanism of toxicity using gene expression profiles. Toxicol Appl Pharmacol, 2001, 175 ( 1 ) : 28.
  • 6Gerhold D, Lu M, Xu J, et al. Monitoring expression of genes unvolved in drug metabolism and toxicology rsing DNA microarrays [JJ. physiol Genomics, 2001, 5 ( 4 ) : 161 -170.
  • 7Pennie WD, Woodyacc N J, Aldridge TC, et al. Application of genomics to the definition of the molecular basis for toxicity[J]. Toxical lett, 2001, 120( 1 - 3):353 - 358.
  • 8Wessendorf S,Fritz B,Wrobel G,et al. Automated screening for genomic imbalances using matrix-based comparative genomic hybridization[J]. Lab Invest,2000,82:47.
  • 9Kononen J ,Bubendorf L ,Kallionieni A ,et al. Tissue microarrays for high- throught put molecular profiling of tumor specimens.Nature Med,1998,4:844～ 847.
  • 10Moch H ,Kononen J , Kallionieni A ,et al. Tissue microarrays:what will they bring to molecular and anatomic pathology? Adv Anat Pathol ,2001,8:14～ 20.

引证文献4

二级引证文献1

中国实验诊断学
2003年 第3期

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