蛋白质芯片及临床应用

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摘要人类基因组排序工作的完成是人类医学史上的里程碑.基因芯片虽可在mRNA水平上分析整个基因组表达的情况,但生命活动的主体是人体内存在的10万种以上的蛋白质,发展蛋白质芯片这一高新技术已成为生物芯片领域的挑战性课题.本文仅对蛋白质芯片的基本原理、临床应用及前景等作简单介绍.

作者单宁宁孙宜山邹雄

机构地区山东大学齐鲁医院检验科山东大学医学院

出处《西北医学教育》 2003年第2期159-162,共4页 Northwest Medical Education

关键词蛋白质芯片临床应用生物芯片基因芯片

参考文献20

1Lin SR,Tseng FG, Huang HM,et al. Mierosized 2D protein arrays immobilized by micro-stamps and micro-wells for disease diagnosis and drug screening. Fresenius J Anal Chem.Sep, 2001,371(2) :202-208.
2Bussow K,Cahill D,Nietfeld W,et al. A method for global protein expression and anitibody sereening on high-density filters of an arrayed cDNA library. Nueleie Aeids Res, 1998, 26(21) :5007 - 5008.
3W. Hutchens,T. Yip, Rapid Commun. Mass Spectrom, 1993,7:69-72.
4Borrebaeck CA, Ekstrom S. Protein chips based on recombinant antibody fragments: a highly sensitive approach as detected by mass spectrometry. Biotechniques, May, 2001,30(5) :1126- 1132.
5G. JIN. P. Tengvall, I. Lundstrom Arwin Anal Biochem.1995,232:69-72.
6Heart disease protein makers first fruit of Large Seale science Vieki Brower, Biotechnology Newswatch, Jul, 2001,2:1.
7Mezzasoma L. Bacarese HT, Di-Cristina M. Antigen microarrays for serodiagnosis of infectious diseases. Clin Chem. Jan, 2002.48(1):121-130.
8Hebestreit HF. Proteomics.. an holistic analysis of nature's proteins, Curr Opin Pharmacol, Oct, 2002,1(5): 513 - 520.
9Dierick JF, Dieu M. Remacle J. Proteomics in experimental gerontology. Exp Gerontol, May, 2002,37(5):721-734.
10RostyC, ChristaL,,KuzdzalS Identification of hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein I as a biomarker for pancreatic ductal adenocarcinoma by protein biochip technology. Cancer Res, Mar. 2002.62 (6) : 1868-1875.

西北医学教育

2003年第2期

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