止眩颗粒剂的质量标准研究

Study on the quality standards for Zhixuan Granule

目的 :研究止眩颗粒剂 (泽泻 ,白术等 )的质量标准。方法 :建立泽泻、白术的薄层色谱鉴别方法和 2 3 乙酰泽泻醇B的高效液相色谱含量测定方法。流动相为乙腈 水 ( 70∶3 0 ) ,检测波长 :2 0 8nm ,样品提取滤过后进行分析。结果 :定性方法能检出泽泻、白术。 2 3 乙酰泽泻醇B的线性范围 99～ 13 88.8ng ,r =0 .9999。平均加样回收率为 10 3 .0 5 % ,RSD为2 .41% (n =6)。结论

Objective: The quality standards for Zhixuan Granule (Rhizoma Alismatis, Rhizoma Atractylodis Macrocephalae, etc.) were studied. Methods: The TLC methods for identification of Rhizoma Alismatis、 Rhizoma Atractylodis Macrocephalae were established. A simple HPLC was established for the determination of 23-acetate alisol B. The mobile phase was acetonitrile-water(70∶30). UV detecting wavelength was at 208nm. Results: Rhizoma Alismatis and Rhizoma Atractylodis Macrocephala could be detected. 23-acetate alisol B showed a linear relationship at the concentration range of 99～1388.8ng, r=0.9999. The average recovery was 103.05% and RSD was 2.41%(n=6). Conclusion: This method is suitable for the quality control of Zhixuan Granule.