逍遥片保肝作用的实验研究

Study of Xiaoyao Tablet on hepatoprotective action

目的 :对逍遥片 (柴胡 ,当归 ,白术 ,白芍 ,茯苓 ,甘草 ,薄荷 ,煨生姜 )进行保肝及镇痛作用的研究。方法 :测定药物对D 半乳糖胺和四氯化碳模型动物血清谷丙转氨酶 (ALT)、谷草转氨酶 (AST)活性 ;对四氯化碳模型小鼠肝组织丙二醛含量和谷胱甘肽S转移酶活性的影响 ;对正常小鼠肝组织丙二醛含量的影响。观察用药后小鼠对热板和冰醋酸致痛的反应性。结果 :逍遥片能显著降低模型动物血清ALT、AST活性 ;降低肝组织中丙二醛的含量、升高谷胱甘肽S转移酶活性 ;还能显著降低正常小鼠肝组织中丙二醛的含量 ;明显减少小鼠扭体反应次数和提高痛阈值。上述作用均与逍遥丸相似。结论

Objective: To study the protective effect of Xiaoyao Tablet(XYT)(Radix Bupleuri, Radix Angelicae Sinensis, Rhizoma Atractylodis Macrocephalae, Radix Paeoniae Alba, Poria, Radix Glycyrrhizae, Herba Menthae, Roasted ginger) on liver. Methods: Using the acute hepatic injury model induced by Tetrachloride and D-galactosamine (CD-GaIN) in rats or mice, we observed the influence of XYT on ALT and AST level in the serum, MDA level and glutathione-s-transferase(GST) activity in the liver homogenates. The pain-killing effect was tested by body twisting model induced by glacial acetic acid and by hot plate model in mice. Results: The XYT reduced the serum ALT level significantly in the acute hepatic injury model in rats and mice. In the CCl 4 reduced acute hepatic injury mice model, XYT decreased the MDA level and increased the GST activity in liver significantly, also it reduced the MDA level in the serum. The XYT reduced twisting number in mice induced by glacial acetic acid. In the hot plate test, the pain threshold was increased. Conclusion: The drug has the similar hepatoprotective function to Xiaoyao Pills.