Ⅱ型糖尿病患者红细胞天然免疫功能变化与CR1密度基因多态性的相关分析

Immune function of erythrocyte and CR1 genomic polymorphism in patients with type Ⅱ diabetes mellitus

目的 研究Ⅱ型糖尿病 (ⅡDM)患者的红细胞CR1密度基因多态性及红细胞免疫粘附功能 ,探讨ⅡDM患者红细胞免疫粘附功能低下的原因。方法 分ⅡDM组和健康对照组 ,用PCR RELP方法测红细胞CR1密度基因多态性 ,红细胞天然免疫粘附活性用红细胞C3b受体花环率(EC3bRR)及免疫复合物花环率 (ECICR)测定。结果 (1)ECR1基因构成 ,ⅡDM组HH型为 5 7.14 %、HL型 37.5 0 %、LL型 5 .36 % ;对照组HH型为 6 8.86 %、HL型 2 8.5 7%、LL型 3.5 7% ;ⅡDM组的基因缺陷率为 4 2 .86 % ,对照组为 32 .14 %。两组相比基因构成及缺陷率差异均无显著性 (P >0 .0 5 )。 (2 )与对照组相比 ,ⅡDM组EC3bRR下降 ,ECICR上升 (P <0 .0 1)。 (3)两组组内比较 ,HH型的EC3bRR均明显高于HL型 ,ECICR均低于HL型。结论 ⅡDM患者红细胞CR1密度基因HH型比率下降不显著 ,但红细胞天然免疫功能低下程度与ECR1基因缺陷关系密切相关。不同ECR1基因型的人群红细胞免疫功能有差异 ,HH型的红细胞免疫粘附功能强于HL型。

Objective To study the frequency of CR1 genomic density polymorphism in patients with type Ⅱ diabetes mellitus (ⅡDM) and their RBC immune function and to find out the possible mechanism. Methods PCR RFLP were used to test the subjects CR1 genomic density polymorphism. RBC adhesion rosette test was used to record RBC CR1 rosette rate and CIC receptor rosette rate (CICR). Results (1) In ⅡDM group was 42.86%, compared with 32.14% in control group, there was no difference in terms of the mutation frequency and HH/HL. (2) Compared with the control groups, the RBC C3bR rate of patients with type ⅡDM was lower while RBC CICR was higher ( P <0.01). Conclusion Patients with type Ⅱ DM were found with lower RBC immune function, which was not associated with the different RBC CR1 density genomic types. The results supported the lower RBC immune function in ⅡDM patients. [