离子束介导外源全DNA转化拟南芥菜的诱变效应

Mutagenic Effect of Exogenous DNA Transformation of Arabidopsis thaliana Mediated by Ion Beam

用 30kev的Ar+ 离子 ,以 1.5× 10 17ions/cm2 的注入剂量分别介导外源拟南芥菜 (Lansberg生态型 )、甘蓝、红甜菜和芦荟的全DNA转化拟南芥菜种子 (Columbia生态型 )。转化当代营养期表型变异率与外源供体和转化受体的亲缘远近没有必然的联系 ,而低育性株率却随着外源供体亲缘关系的从近到远 ,呈现一种从低到高再变低的变化趋势。从低育性株后代中选育出 2个稳定的突变体 ,该突变体不但保持了其亲本的低育性 ,而且其他性状也有较显著的变异 ,如株型、叶型等。

The total DNAs of A. thaliana (Lansberg ecotype), cabbage, red beet and aloes were respectively transformed into the A. thaliana (Columbia ecotype), mediated by Ar + ion beam with the energy of 30 Kev and influence of 1.5×10 17ions/cm 2. In transferred generation, the rate of low-fertility plant changed from low to high, then to low with genetic relationship between recipient and DNA donors decreasing, while the rate of phenotypic variation in vegetal period had no link to genetic relationship. We analyzed the possible reason from the viewpoint of structure and development of A. thaliana's mature embryo and features of ion-mediated transformation. Two stable mutants were obtained from offspring of low-fertility plants which not only kept the low-fertility but produced other phenotypic variations such as plant type and phylliform.