ST-1抗兔心缺血复灌作用药效学研究

Effects of ST-1 on myocardial dysfunction induced by ischemia and reperfusion in anesthetized rabbits

目的 证实双萜类化合物ST 1抗兔心缺血 /复灌损伤作用。方法 麻醉下结扎兔冠状动脉左前降支 30min后 ,再连续灌注 60min ,造成心肌缺血再灌注损伤模型。动态观察血流动力学指标 :LVSP ,LVDP’±dp/dtmax ,T dp/dtmax ,左室发展压 (leftventriculardevelopmentalpressure,LVDP’) ,LVEDP ,HR ,MAP ,SAP ,DAP。实验分伪手术组、溶剂对照组和ST 1高、中、低 3个剂量给药组和氨氯地平阳性药对照组。结果 在整个缺血复灌期 ,ST 1组±dp/dtmax ,LVSP ,LVDP’ ,T dp/dtmax的变化值明显小于溶剂对照组 (P <0 .0 5) ;HR ,SAP ,DAP和MAP变化值与溶剂对照组之间无显著性差别 (P >0 .0 5)。结论 ST 1预处理可明显改善兔心缺血 /复灌引起的左室舒缩功能的损伤。同时无扩血管作用 。

OBJECTIVE: To study the effects of ST-1, a diterpene compound, on myocardial dysfunction induced by ischemia/reperfusion. METHODS: The changes of hemodynamics, such as LVSP, LVDP' (left ventricular development pressure), ± dp/dt max, T-dp/dt max, LVDP, LVEDP, HR, MAP, SAP, DAP were determined in rabbits with myocardial ischemia/reperfusion model induced by occluding the left anterion descending coronary artery (LAD) for 30 min followed by 60 min reperfusion. The rabbits were divided into four groups: control, amlodipine (0. 3 mg&middotkg-1, iv), ST-1 (100, 50 and 25 μg&middotkg -1, iv) and sham operation groups respectively. RESULTS: The values of change in LVSP, LVDP' ± dp/dt max, T-dp/dt max induced by ST-1 and amlodipine were significantly less than the respective values in the anesthetized, ischemia/reperfused rabbits. There were no significant changes on heart rate and blood pressure by administration of ST-1, but a significant hypotension was induced by amlodipine. CONCLUSION: ST-1 ameliorated cardiac dysfunction induced by ischemia and reperfusion without influence on heart rate and arterial blood pressure in anesthetized rabbits.