摘要
目的 :探讨热休克蛋白 70在人类糖性白内障晶状体上皮细胞中的表达及意义。方法 :采用链霉菌抗生物素蛋白碱性磷酸酶 (streptavidin alkalinephosphatase ,S P)免疫组化技术检测热休克蛋白 70 (heatshockprotin 70 ,HSP 70 )在人类糖性白内障 (2 5例 )和正常晶状体 (7例 )上皮细胞中的表达情况。结果 :热休克蛋白 70在人类糖性白内障晶状体上皮中全部为阳性表达 ,而在正常晶状体上皮细胞中全部为阴性表达 ,二者差异有显著性 (χ2 =2 6 .4 2 ,P <0 .0 1)。结论 :热休克蛋白 70在人类糖尿病性白内障发生、发展中起重要作用。
Objective:To investigate the expression and significance of HSP 70(heat shock protin 70) in human diabetic cataract lens epithelial cells(LECs).Methods:The expression of HSP 70 was assayed using immunohistochemistry(streptavidin alkaline phosphatase,S P) in human diabetic cataract LECs(25 cases) and human normal LECs(7 cases).Results:There was significant expression of HSP 70(χ 2=26.42,P<0.01) in human diabetic LECs(25 cases) but there was no expression of HSP 70 in human normal LECs(7 cases).Conclusion:HSP 70 may play a critical role during the development and formation of human diabetic cataract.[
出处
《眼视光学杂志》
CAS
2003年第2期72-74,共3页
Chinese Journal of Optometry & Ophthalmology
基金
福建省自然科学基金资助项目 ( 2 0 0 1Z0 3 3 )
