摘要
目的 探讨给予大鼠不同剂量“抗纤复方”药物后其血清对体外培养正常大鼠肝细胞增殖与活力和纤维肝大鼠肝细胞功能表达的影响。方法 常规培养大鼠肝细胞 ,用 5、10、2 0倍剂量“抗纤复方”药物血清分别温育肝细胞 48h ,同时以对照血清作为参照 ,用3H TdR掺入法测定细胞增殖 ,3H Pro掺入法测定细胞活力 ,用PAS法染色糖原 ,用偶氮偶联法染色碱性磷酸酶。结果 各剂量组“抗纤复方”药物血清均能增加肝细胞3H TdR(2 0 %的 2 0倍剂量组除外 )和3H Pro掺入量 ,且“抗纤复方”具有促进纤维肝肝细胞活力与糖原合成、抑制纤维肝肝细胞增殖与碱性磷酸酶活性的作用。结论 “抗纤复方”药物血清能促进正常肝细胞增殖和活力 。
Objective: To study the effect of drug sera of anti?fibrosis compond priscription (Acp)on the functions of primarily cultured normal and fibrous hepatocyte such as proliferation and viability et. Methods: Primarily cultured normal and fibrous hepatocytes were cultured with the regular method and incubated with 5, 10, 25 times drug sera of Acp for 48 h. The cell proliferation was measured by 3H?TdR incorporation, the cell viability by 3H?Pro line incorporation and cell glycogen by periodic acidschiff reaction. Results: The three drug sera groups could promote the proliferation and viability of normal hepatocytes, xpromote the viability and synthetic glycogen of fibrous hepatocytes and inhibit the proliferation of fibrous hepatocyted. Conclusion: The Acp could enhance the proliferation and viability of normal hepatocytes and regulate the abnormal metabolism and function of hepatocys.
出处
《实用临床医药杂志》
CAS
2003年第2期138-141,共4页
Journal of Clinical Medicine in Practice
关键词
抗纤复方
药物血清
体外培养
大鼠
肝细胞功能
表达
影响
cirrhosis
hepatocyte proliferation
heptocyte viability
Glycogen
alkaline phosphorase
rats
in vitro