丙泊酚对大鼠全心缺血-再灌注损伤后心肌细胞凋亡的影响

Effects of propofol on myocardial apoptosis during global myocardial ischemia-reperfusion in rat

目的 探讨丙泊酚对大鼠全心缺血 再灌注损伤后心肌细胞凋亡的影响。方法 Wis tar大鼠 36只 ,随机分为对照组 (C组 )和丙泊酚组 (P组 )。每组再分为缺血前、缺血 30min和再灌注30min三个亚组。采用DNA原位末端缺口标记技术 (TUNEL法 ) ,观察各组心肌细胞凋亡的变化情况。结果 与缺血前组相比较 ,缺血 30min及再灌注 30min各组的凋亡指数显著增加 (P <0 0 1) ,而丙泊酚组中缺血 30min及再灌注 30min各亚组的凋亡指数 ,均较对照组显著降低 (P <0 0 1)。结论 缺血 30min及再灌注 30min的心肌均发生了细胞凋亡 ,且再灌注组的细胞凋亡更为显著。丙泊酚可减少心肌缺血 再灌注时心肌细胞凋亡的发生。

Objective To study the effects of propofol on apoptosis during global myocardial ischemia-reperfusion injury.Methods Thirty-six Wistar rats were randomly divided into control group and propofol group,which were perfused with pure K-H perfusate and 10μmol/L propofol respectively.Each group was redivided into 3 subgroups:preischemia,ischemia 30min and ischemia-reperfusion 30min group.Myocardial apoptosis was identified by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL).Results The TUNEL results showed that the apoptosis index in ischemia 30min group and ischemia-reperfusion 30min group increased significantly compared to that in preischemia group(P<0.01),and the increase in reperfusion group was more obviously(P<0.01).The apoptosis index of propofol group decreased compared to that in control group(P<0.01)at ischemia 30min and ischemia-reperfusion 30min.Conclusions The present study shows that either ischemia 30min or ischemia followed by reperfusion 30min can cause apoptosis in isolated rat model of Langendorff perfused,and the apoptosis phenomenon in reperfusion group is more obviously.Propofol can reduce the occurrence of apoptosis.