HPLC法测定氢溴酸加兰他敏分散片的含量

Determination of Galanthamine Hydrobromide Dispersion Tablet by HPLC

目的 :建立氢溴酸加兰他敏及其分散片的HPLC分析方法。方法 :采用ODS色谱柱 (15 0mm× 4 .6mm ,5 μm) ,以甲醇 水 冰醋酸 (75∶92 5∶10 )为流动相 ;流速 :1.0ml·min-1,检测波长 :2 89nm ;采用外标法测定氢溴酸加兰他敏分散片的含量。结果 :氢溴酸加兰他敏在 10～ 10 0 μg·ml-1范围内 ,线性关系良好 (r =0 .9998) ;平均回收率为 10 0 .2 % ;日内精密度(RSD =0 .6 % ,n =5 )良好。结论 :本法适用于氢溴酸加兰他敏及其分散片的含量测定 ,方法简便、准确、快速。

Objective: To establish a method of HPLC for determination of galanthemine hydrobromide dispersion tablets.Methods: A ODS column(150 mm× 4.6 mm, 5 μm)was used. The mobile phase consisted of methanol water acetic acid(75∶925∶10), the flow rate was 1.0 ml·min -1 and the detective wavelength was at 289 nm. Calculate the content of galanthamine hydrobromide by the external standard.Results: The linear correlation of galanthamine hydrobromide with a range of 10 100 μg·ml -1 ( r = 0.9998 ). The average recovery was 100.2% . The precision in a day ( RSD = 0.59% ) was good.Conclusion: This method may be used in the analysis of galanthamine hydrobromide, it is accurate, simple, and rapid.