高效液相色谱法测定维磷颗粒中咖啡因的含量

Content Determination of Caffeine in Weilin granules by HPLC

目的 :建立高效液相色谱法测定维磷颗粒中咖啡因的含量。方法 :采用ODS柱 ,甲醇 水 (30∶70 )为流动相 ,检测波长为 2 72nm。结果 :咖啡因在 0 .14 6～ 1.16 8μg范围内有良好的线性关系 (r =0 .9999) ,平均回收率为 99.9% (RSD =0 .2 3% )。结论 :本法快速、简便、准确。

Objective: To determine the content of caffeine in Weilin granules by HPLC. Method: ODS colunm was used, menthanol water(30∶70) was used as mobile phase, the detecting wavelength was at 272 nm by UV detector. Result: Caffeine presented a good linear relation within a range of 0.146 1.168 μg, the arerage recovery was 99.9% . Conclusion: This method is rapid, simple, and accurate.