高效液相色谱法-蒸发光散射检测器测定卡托普利片中卡托普利的含量

Determination of Captopril in Captopril Tablets by HPLC-ELSD

目的 利用高效液相色谱法 -蒸发光散射检测器测定卡托普利 〔1〕片中卡托普利的含量。方法 色谱柱 :RP C1 8柱 ( 4 .6× 3 0 0 mm,5μm) ;流动相 :9%四氢呋喃甲醇溶液∶水 ( 4 0∶ 60 ) (用冰醋酸调节 p H至 3 .0 0 ) ;流速 :1.0 ml· min- 1 ;柱温 :室温 ;以 ELSD-2 0 0 0为检测器。 结果 卡托普利进样量在 13 .4～ 13 4.0 μg范围内 ,线性关系良好 ( r=0 .9915 ) ,平均回收率为 98.8% ,RSD=0 .8% ( n=6)。 结论 该法简便 ,快速 ,结果准确、可靠 ,重现性好。

OBJECTIVE To establish a method HPLC ELSD for the determination of Captopril in Captopril Tablets.METHODS The Chromatographic conditions included RP C 18 Column(4 6×300mm,5μm);the mobile phase was 9% Tetrahydrofuran of Methaol∶water(40∶60)(pH3 00);the flow rate 1 0ml·min -1 and column temperature at room temperature.RESULTS The calibration curve was good linear relationship in the range of 13 4～134 0μg(r=0 9915).The recovery was 98 8%,RSD=0 8%(n=6).CONCLUSION The method is simple and rapid,the results is accurate and good reproducible.