摘要
目的 建立一种高效液相色谱法测定 7-氨基头孢烷酸含量及其有关物质的方法。 方法 采用 Merck公司的 C1 8色谱柱 ( 4 .6mm×2 5 0 mm,5 μm) ,以 0 .0 2 mol· L- 1醋酸钠 ( p H=5 .5 ) -乙腈 ( 93 0∶ 70 )为流动相 ,流速 0 .7m L· min- 1 ,检测波长 2 63 nm,柱温 3 5℃ ,样品池温度8℃。 结果 线性范围为 40～ 3 60 μg·m L- 1 ,r=0 .9999,平均回收率为 10 0 .86%。 结论 本方法简便、快速。
OBJECTIVE A HPLC method was established for the determination of 7 Arninocophalosporacic Acid and it related substances.METHOD\ The chromatographic conditions were as follows:Merck C 18 column(4 6mm×250mm,5μm),0 02mol.L -1 sodium acetate(pH=5 5) and acetontrile(930∶70) as mobile phase,at a flow rate of 0 7mL.min -1 ,at detective wavelength 263nm,tempeture of the column was 35℃,temperature of the sample cell was 8℃.RESULTS\ The standard curve was linear in the range of 40～360μg.mL -1 and the correlation coefficient was 0 9999.The average recovery was 100 86%.CONCLESION\ This method is simple,rapid, and accurate.
出处
《海峡药学》
2003年第3期16-18,共3页
Strait Pharmaceutical Journal
