糖尿病肾病患者血清PON活性变化及其临床意义

Association of paraoxonase activity with diabetic nephropathy

目的:探讨对氧磷酯酶(PON)活性变化与糖尿病肾病(DN)的关系。方法:分光光度法检测实验对象血清PON活性;放免法检测血清免疫反应性胰岛素(IRI)C肽(C-P)水平。结果:DN患者、2型糖尿病患者(T2D)、正常对照者血清PON活性平均值是上升趋势,即PON活性按以下顺序增高:ON<T2D<正常对照者。相关分析结果显示PON活性与空腹及餐后2h血清C-肽水平无明显相关(r=0.112,P>0.05;r=0.138 P>0.05),而与血清低密度脂蛋白成负相关(r=-0.159,P<0.05)。Logistic回归分析表明:PON活性(P<0.001,模型总判对率93.7％)、LDL(P<0.001,模型总判对率89.4％)是DN的独立变异危险因素。结论:PON活性变化与DN的发病有关。

Objective: To investigate the association of the variation of paraoxonase ( PON) activity with Diabetic Nephropathy ( DN ) , and the pathogenesis of DN. Methods: PON activity was determined with spectrophotography in DN patients, type Ⅱdiabetes (T2D) patients and healthy volunteers as the control group. Serum immune -reactive insulin (IRI) and C - peptides (C -P) were measured with radioimmunoassay method. Results: PON activities in DN patients, T2D patients, and healthy volunteers increased in the sequence of DN < T2D < control. Correlation analysis results suggested that PON activity was not correlated with fasting and postprandial C -peptide level (r = 0.112, P >0. 005; r = 0. 138, P >0. 005) , however, it was correlated with serum LDL (r= -0. 159, P < 0. 005 ). Logistic regression analysis suggested that PON activity (P < 0. 001 ) and LDL ( P < 0. 001 ) were the independent risk factors of DN. Conclusion: PON activity is associated with the pathogenesis of DN.