SARS病毒在外界环境物品中生存和抵抗能力的研究

SURVIVAL STUDY OF SARS VIRUS IN VITRO

评估SARS病毒离开人体以后在外界环境物品中生存的能力.采用的方法是将滴度为106]TCID#-的SARS病毒BJ-01株涂布在灭菌的不锈钢片、玻璃片、塑料片、滤纸片、棉布片和木片的表面,或混合在灭菌自来水和土壤样品中,经一定时间后洗下病毒,接种Vero-E6细胞,观察细胞病变.结果,SARS病毒在模拟污染的不锈钢片、玻璃片、塑料片上可以存活至少2 d,在模拟污染的棉布片、土壤、滤纸片和木片上至少可存活4～6 h,在污染的自来水中2 d仍然保持较强的感染性.干燥是影响SARS病毒生存时间的重要因素.结论,SARS病毒离开人体以后在外界环境物品中具有较强的生存能力,做好对环境物品的消毒对于切断传播途径十分重要.

Objective Evaluate the survival ability of SARS virus in vitro. Method: Smearing 106 TCID50 SARS virus BJ-01 strain on the surface of sterilized metal slice, glass slice, plastics slice, paper slice ,fabric slice and wood slice, adding the virus to sterilized water and soil. Rinsing and infecting Vero-E6 cell after definite time points. Result: SARS virus keep infectious at least 48 hr in the analogue contaminated metal slice, glass slice and plastics slice, the infectious titer reduced about 5 log with 48 hours. The virus can survival at least 6hr in the analogue contaminated paper slice, fabric slice, wood slice. The virus is very stable in tap water at least 2 d. Conclusion: SARS virus can survival long time after discharge outside human body. It is very important sterilizing contaminated objects.