食管癌患者3p25等位基因杂合缺失的初步研究

Detection of allele-specific chromosome 3p25 by PCR -LOH in 35 cases of esophageal carcinoma

目的 :检测 35例食管癌患者 3p2 5 D3S10 38染色体位点的杂合缺失 ,为寻找此位点附近潜在的抑癌基因奠定基础。 方法 :微卫星 DNA- PCR-银染法检测 3p2 5 D3S10 38位点杂合缺失。结果:3p2 5 D3S10 38位点有 11例存在杂合缺失 (L OH) ,L OH率为 31.4 % ,此位点杂合缺失在各民族中均存在。结论:3p2 5 D3S10 38存在杂合缺失且有一定发生频率 ,可能与食管癌的发生有关 ,值得继续研究。

Objective: To study loss of heterozygosity at chromosome 3p25 in 35 patients with esophageal carcinoma. Methods: The loss of heterozygosity at 3p25 was conducted by using microsatellite DNA PCR silver staining method.Results: The rate of LOH at 3p25D3S1038 was 31.4%.Conclusion: There is alteration of microsatellite marker on chromosome 3p25, which is valuable and worthy to study further.