脑瘫仔兔血清及脑组织中神经元特异性烯醇化酶含量的研究

Levels of Neuro-specific Enolase in Serum and Brain Tissues in Newborn Rabbits with Cerebral Palsy

目的 :探讨神经元特异性烯醇化酶 (NSE)能否作为脑瘫早期诊断及反映各脑区损伤情况的生物学指标。方法 :对生后 3d仔兔腹腔分次注射胆红素 (总量 30 0mg/kg)制备出脑瘫兔模型 ,并观察脑组织不同区域病理变化 ;采用酶联免疫法测定血清和脑组织中NSE含量。结果 :脑瘫兔 (实验组 )血清中NSE含量末次注药后 12h较对照组明显升高 (P <0 .0 5 ) ,且神经元数量减少 ,而 4 5d后实验组与对照组比较差异无显著性。实验组海马、基底节区NSE含量较对照组减少 (P <0 .0 5 ) ,而脑干、小脑、皮层区NSE含量与对照组比较差异无显著性 (P >0 .0 5 )。结论 :血清中NSE含量可作为胆红素致脑损伤及脑瘫早期诊断的敏感指标 ;胆红素致脑瘫兔海马、基底神经节区神经元数量减少。

Objective:To study if neuro-specific enolase (NSE) can be used as a biological marker for early diagnosis and damage in different brain regions of cerebral palsy.Methods:The model of cerebral palsy was established by intraperitoneal injection of bilirubin (300 mg/kg) in the rabbits of postnatal 3 days. The pathological changes of different brain regions were observed. The levels of NSE in serum and brain tissues were measured. Results:The levels of NSE in cerebral palsy newborn rabbits at 12 h after last administration were significantly higher than those in the control group( P <0.05), but there was no difference in the levels of serum NSE between cerebral palsy newborn rabbits at 45 days after last administration and those in the controls. The contents of NSE in hippocampus and basal ganglia in cerebral palsy newborn rabbits were reduced, but there were no changes of NSE in brainstem, cerebellum. Conclusion:The levels of serum NSE can be used as a sensitive marker for the early diagnosis of bilirubin-induced brain damage and cerebral palsy. The number of neurons was decreased in hippocampus, basal ganglia in the rabbits with bilirubin-induced cerebral pasly.