河北省大豆种质资源同工酶及RAPD标记多样性研究

Genetic diversity study of soybean germplasm resource in Hebei province by isozyme and RAPD

利用过氧化物酶(POD)同工酶和RAPD分子标记技术对60份河北省大豆种质资源的遗传多样性进行分析。结果表明,过氧化物酶同工酶谱带有12条,酶谱类型有21种,聚类结果基本上能够区分不同生态区的材料。RAPD分子标记多态性高,10个引物扩增出58条谱带,29条具有多态性,聚类结果可将供试材料划分为8个类群,揭示了其亲缘关系,同时,还反映出品种或品系与地理起源有一定的相关性。

The genetic diversity of 60 soybean germplasm resources in Hebei were analyzed by POD isozyme and RAPD molecular markers. The result showed that there were 12 bands of POD isozyme and 21 kinds of zymogram styles. The materials from ecological regions could be plotted out by this method. Total 58 RAPD bands obtained from 10 primers. Among them 29 were ploymorphic bands. The tested materials could be divided into 8 groups. RAPD marker indicated better results than POD isozyme for revealing genetic background and consanguinity. The cluster analysis of varieties and lines exhibited their relation to the geographic origin.