摘要
以聚乙烯醇复合凝胶作为载体固定化糖化酶 ,最终酶活达到 1,5 5 8u/ g干胶 ,酶活回收率是 30 2 %。该固定化酶在 pH4 6 ,4 5℃下 ,以 10 %的可溶性淀粉为底物进行分批试验 ,操作半衰期为 35 0h 。
In this article, the Glucoamylase was immobilized onto the polyvinyl alcohol complex gel The results showed that the activity of immobilized Glucoamylase was 1,558 U per gram dry gel,the activity recovery was 30 2% The enzyme system could directly converted 10% starch hydrolyses to glucose under the conditions of pH4 6 and 45℃ The results showed that the working half life of immobilized Glucoamylase was 350 hours The immobilized Glucoamylase has excellent store and working stability
出处
《微生物学通报》
CAS
CSCD
北大核心
2003年第3期10-13,共4页
Microbiology China
基金
甘肃省中青年科学基金资助项目(No .YS991 A2 2014 )
关键词
聚乙烯醇复合凝胶
固定化
糖化酶
Polyvinyl alcohol Complex gel,Immobilization,Glucoamylase
